To produce genetically engineered livestock, many enabling technology developments have been required.\u00a0 The previous or current members of the MULE Team have led the field, world-wide, with many of the \u201cfirsts\u201d in regard to embryo technologies or livestock engineering.\u00a0 Some of these \u201cfirsts\u201d include the following:<\/p>\n
First pig zygotes reconstructed from transplanted nuclei (Prather et al ’89);<\/p>\n
Identification of when the pig zygotic genome is first activated (Jarrell et al ’91);<\/p>\n
Development of the first robust porcine embryo culture system (Petters & Wells ’93);<\/p>\n
Development of the first robust chemical method of porcine oocyte activation (Machaty et al ’97) (a revenue generating IP);<\/p>\n
First robust in vitro<\/em> fertilization system for pigs, (Abeydeera & Day ’97);<\/p>\n Development of the first robust system for porcine oocyte maturation (Wang et al ’97);<\/p>\n Production of the first genetically engineered pig model for a human condition (Petters et al ’97):<\/p>\n Production of the first genetically engineered livestock expressing an agriculturally relevant trait (Wall et al ’05);<\/p>\n First seven examples of gene knockouts in the pig (Lai et al ’02; Lorson et al ’11; Mendicino et al ’11; Prather et al ’13; Ramsoondar et al ’11; Rogers et al ’08a; Rogers et al ’08b) (the IP described in Lai et al \u201902 continues to generate revenue);<\/p>\n The first pigs that transgenically express microRNAs (Ramsoondar et al ’09);<\/p>\n The first gene knockout in a pig from gene editing (Whyte et al ’11);<\/p>\n The first three pig knockouts from CRISPR\/Cas9 technology (Whitworth et al ’14);<\/p>\n The first domain swap in pigs (Wells et al ’17);<\/p>\n The first CRE deletion in pig zygotes (Whitworth et al ’18);<\/p>\n and the first gene edited pig to be licensed for commercial use (Whitworth et al ’16).<\/p>\n Additionally, the group developed an inexpensive alternative to commercial sources of Cas9 (Redel et al ’18).<\/p>\n","protected":false},"excerpt":{"rendered":" To produce genetically engineered livestock, many enabling technology developments have been required.\u00a0 The previous or current members of the MULE Team have led the field, world-wide, with many of the \u201cfirsts\u201d in regard to embryo technologies or livestock engineering.\u00a0 Some of these \u201cfirsts\u201d include the following: First pig zygotes reconstructed from transplanted nuclei (Prather et […]<\/p>\n","protected":false},"author":13,"featured_media":90,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-20","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/pages\/20","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/comments?post=20"}],"version-history":[{"count":4,"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/pages\/20\/revisions"}],"predecessor-version":[{"id":66,"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/pages\/20\/revisions\/66"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/media\/90"}],"wp:attachment":[{"href":"https:\/\/cafnrfaculty.missouri.edu\/muleteam\/wp-json\/wp\/v2\/media?parent=20"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}