Research Scientist
Education & Positions Held
B.S. in Biochemistry 06/2007; Charles University in Prague, Czech Republic
Graduate student/Lab assistant 2008-2014; Institute of Biotechnology CAS, v.v.i. in Vestec, Czech Republic
M.S. in Biochemistry 06/2009; Charles University in Prague, Czech Republic
B.B.A. in Business Administration and Management 06/2012; University of Economics in Prague, Czech Republic
M.B.A. in Business Administration and Management 09/2014; University of Economics in Prague, Czech Republic
PhD in Biochemistry/Reproductive biology 11/2014; Charles University in Prague
Postdoctoral Fellow 2014-2019 ; University of Missouri, Columbia, MO
Research Scientist 2020-present; University of Missouri, Columbia, MO
Awards & Honors:
2019 University of Missouri MU Postdoctoral Association Travel Award.
2018 Larry Ewing Memorial Trainee Travel Award, Presented at 51th Annual Meeting of the Society for Study of Reproduction, 10-13 July 2018 in New Orleans, LA.
2017 University of Missouri Postdoctoral Association Travel Award to attend 50th Annual Meeting of the Society for Study of Reproduction, 13-16 July 2017 in Washington D.C.
2017 Larry Ewing Memorial Trainee Travel Award, Presented at 50th Annual Meeting of the Society for Study of Reproduction, 13-16 July 2017 in Washington D.C.
2017 USDA-NIFA-AFRI Merit Award, Presented at 50th Annual Meeting of the Society for Study of Reproduction, 13-16 July 2017 in Washington D.C.
2016 Larry Ewing Memorial Trainee Travel Award made by the Society for The Study of Reproduction, Presented at 49th Annual Meeting of the Society for Study of Reproduction, 16-20 July 2016 in San Diego, CA.
2016 Zigo M: DNA Fragmentation as a Cause of Infertility, Invited lecture at the 2016 Gateway to Parenthood St. Louis Infertility Awareness, St. Louis 30 April 2016
2015 Nadace “Nadání Josefa, Marie a Zdeňky Hlávkových” (Hlavkas’ Talent Foundation) travel stipend for moving to Columbia, MO to start my postdoc.
2010 Nadace Český Literarní Fond (The Czech Literary Fund Foundation) travel stipend to attend 6th European Congress of Andrology, 29 September – 1 October 2010 in Athens, Greece.
2010 Nadace “Nadání Josefa, Marie a Zdeňky Hlávkových” (Hlavkas’ Talent Foundation) travel stipend to attend 6th European Congress of Andrology, 29 September – 1 October 2010 in Athens, Greece.
Peer-reviewed Publications
Chen PR, Lucas CG, Cecil RF, Pfeiffer CA, Fudge MA, Samuel MS, Zigo M, Seo H, Lee D. Spate LD, Whitworth KM, Sutovsky P, Wells KD, Geisert RD, Prather RS (2021) Disrupting porcine glutaminase does not impair preimplantation development and elongation nor decrease mTORC1 activation. Biol. Reprod., In press.
Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2021) Ligands and receptors involved in the sperm-zona pellucida interactions in mammals. Cells, In press.
Mao J, Zigo M, Zuidema D, Sutovsky M, and Sutovsky P (2021) NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization. Biol. Reprod., 104(1):117-129.
Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2020) Is the ubiquitin-proteasome system involved in the degradation of porcine beta-microseminoprotein during sperm capacitation? Int. J. Mol. Sci., 21(11):4151.
Zigo M, Maňásková-Postlerová P, Zuidema D, Kerns K, Jonáková V, Tůmová L, Bubeníčková F, Sutovsky P (2020) Porcine model for the study of sperm capacitation, fertilization and male fertility. Cell Tissue Res., 380(2):237-262.
Hamilton LE, Zigo M, Mao J, Xu W3, Sutovsky P, O’Flaherty C, Oko R (2019) GSTO2 isoforms participate in the oxidative regulation of the plasmalemma in eutherian spermatozoa during capacitation. Antioxidants 8: 601.
Kelsey KM, Zigo M, Thompson WE, Kerns K, Gaurishankar Manandhar G, Sutovsky M, Sutovsky P (2019) Reciprocal surface expression of arylsulfatase A and ubiquitin in normal and defective mammalian spermatozoa. Cell Tissue Res., 379:561-576.
Zigo M, Manaskova-Postlerova P, Jonakova V, Kerns K, Sutovsky P (2019) Compartmentalization of the proteasome-interacting proteins during sperm capacitation. Sci. Rep. 9:12583
Zigo M, Jonáková V, Maňásková-Postlerová P, Kerns K, Sutovsky P (2019) Ubiquitin-proteasome system participates in the de-aggregation of spermadhesins and DQH protein during boar sperm capacitation. Reproduction 157:283-95.
Mordhorst B, Kerns K, Schauflinger M, Zigo M, Murphy S, Ross R, Wells K, Green J, Sutovsky P, Prather R (2019) Pharmacologic treatment with CPI-613 and PS48 decreases mitochondrial membrane potential and increases quantity of autolysosomes in porcine fibroblasts. Sci. Rep. 9:9417.
Sutovsky P, Kerns K, Zigo M, Zuidema D (2019) Boar semen improvement through sperm capacitation management, with emphasis on zinc ion homeostasis. Theriogenology, 137:50-55
Kerns K, Zigo M, Sutovsky P (2018) Zinc: A Necessary Ion for Mammalian Sperm Fertilization Competency (Review). Int J Mol Sci 19:E4097
Mao J, O’Gorman C, Sutovsky M, Zigo M, Wells KD, Sutovsky P (2018) Ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52) is essential for preimplantation embryo development. Biol Open 7:bio035717
Kerns K, Zigo M, Drobnis EZ, Sutovsky M, Sutovsky P (2018) Zinc ion flux during mammalian sperm capacitation. Nat Commun 9:2061
Zigo M, Kerns K, Sutovsky M, Sutovsky P (2018) Modifications of the 26S proteasome during boar sperm capacitation. Cell Tissue Res 372:591-601
Zigo M, Dorosh A, Pohlová A, Jonáková V, Šulc M, Maňásková-Postlerová P (2015) Panel of monoclonal antibodies to sperm surface proteins as a tool for monitoring localization and identification of sperm-zona pellucida receptors. Cell Tissue Res 359:895-908
Zigo M, Jonáková V, Šulc M, Maňásková-Postlerová P (2013) Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates. Int J Biol Macromol 61:322-328
Zigo M, Jonáková V, Maňásková-Postlerová P (2011) Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms. Electrophoresis 32:1309-18.
Major scientific meeting abstracts
Zigo M, Maňásková-Postlerová P, Jonáková V, Sutovsky P: Ubiquitin-proteasome system (UPS) regulates spermadhesin release during boar sperm capacitation. Presented at 51th Annual Meeting of the Society for Study of Reproduction, 10-13 July, 2018 in New Orleans, LA, and 11th Biennial Conference of Association for Applied Animal Andrology, 14-16 July, 2018 in New Orleans, LA.
Zigo M, Kerns K, Sutovsky M, Sutovsky P: New insights into the involvement of ubiquitin-proteasome system in boar sperm capacitation. Presented at 50th Annual Meeting of the Society for Study of Reproduction, 13-16 July 2017 in Washington D.C.
Zigo M, Kerns K, Sutovsky M, Sutovsky P: Growing evidence of ubiquitin-proteasome system (UPS) involvement in boar sperm capacitation. Presented at 10th International Conference on Pig Reproduction, 11-14 June 2017 in Columbia, MO.
Zigo M, Kerns K, Sutovsky M, Sutovsky P: Pilot studies on the involvement of ubiquitin-proteasome system in mammalian sperm capacitation. Presented at 49th Annual Meeting of the Society for Study of Reproduction, 16-20 July 2016 in San Diego.
Zigo M, Dorosh A, Pohlová A, Jonáková V, Šulc M, Maňásková-Postlerová P: Panel of monoclonal antibodies to sperm surface proteins as tool for monitoring of sperm-zona pellucida receptors localization and identification. Presented at 3rd World Congress on Reproductive Biology, 2-4 September 2014 in Edinburgh, Scotland.
Zigo M, Dorosh A, Jonáková V, Maňásková-Postlerová P: Sperm surface proteins: their isolation, characterization and binding study with zona pellucida. Presented at 2nd World Congress on Reproductive Biology, 9-12 October 2011 in Cairns, Queensland, Australia.
Zigo M, Jonáková V, Maňásková-Postlerová P: Isolation of proteins from ejaculated and capacitated sperms by various extraction approaches. Presented at 6th European Congress of Andrology, 29 September – 1 October 2010 in Athens, Greece. Contribution was published in Int. J. Androl. 33 (2010), suppl. 1, 68-68.
Zigo M, Jonáková V, Maňásková-Postlerová P: Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins. Presented at 3rd Central and Eastern European Proteomics Conference, 6-9 October 2009 in Budapest, Hungary.