Sun F, Sutovsky P, Patterson AL, Balboula AZ (2023) Mechanisms of DNA damage response in mammalian oocytes. Advan. Anat. Embryol. Cell Biol, In press.

Sanches Silva c, Vianna da Costa-e-Silva E, Alves Nunes Dode M, Thainar Mendes Cunha A, Rodrigues Garcia W, Fernandes Barreto Sampaio B; Correa Borges Silva J, Machado Vaz FE, Kerns K, Sutovsky P, Nogueira E (2023). Semen quality of nellore and angus bulls classified by fertility indexes and relations with field fertility in fixed time artificial insemination. Theriogenology, In press.

Zuidema D, Jones A, Song W-H, Zigo M, Sutovsky P (2023) Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system. eLife 12:RP85596. doi: 10.7554/eLife.85596.

Soley J, du Plessis L, Sutovsky M, Sutovsky P (2023) Steps of spermiogenesis in the ostrich (Struthio camelus. Cell Tissue Res., doi: 10.1007/s00441-023-03807-0

Zigo M, Kerns K, Sutovsky P (2023) The ubiquitin-proteasome system participates in sperm surface subproteome remodeling during sperm capacitation. Biomolecules, 13(6):996. doi: 10.3390/biom13060996.

Hackerova L, Klusackova B, Zigo M, Zelenkova N, Havlikova K, Krejcirova R, Sedmikova M, Sutovsky P, Komrskova K, Postlerova P, Simonik O (2023) Modulatory effect of MG-132 proteasomal inhibition on boar sperm motility during in vitro capacitation. Frontiers Vet. Med., 10:1116891 doi: 10.3389/fvets.2023.1116891.

Kern C, Wu W, Lu C, Zhang J, Zhao Y, Ocon-Grove OM, Sutovsky P, Diaz F, Liu W (2023) Role of the bovine PRAMEY protein in sperm function during in vitro fertilization (IVF). Cell Tissue Res., 391:577-594.

Spooner M, Kerns K, Zigo M, Sutovsky P, Balboula A, Patterson AL (2023) A re-appraisal of mesenchymal-epithelial transition (MET) in endometrial epithelialization. Cell Tissue Res. 391:393-408


Sutovsky P (2022) Foreword. In: Oxidative Stress and Toxicity in Reproductive Biology and Medicine – A Comprehensive Update on Male Infertility VOLUME 2, Roychoudhury S and Kesari K, Eds. Springer Media LLC.

Sutovsky P, Hamilton LE (2022) What should I know about artificial insemination of animals? How is male fertility assessed in domestic animals and why is this important? In: Handbook of Andrology 3rd Edition, Robaire B, Chan P, Editors, American Society of Andrology Inc.

Lawlor M, Zigo M, Kerns K, Cho I, Easley CA, Sutovsky P (2022) Spermatozoan metabolism as a non-traditional model for the study of Huntington’s disease. Int. J. Mol. Sci. 23: 7163. DOI: 10.3390/ijms23137163

Liman MS, Hassen A, McGaw LJ, Sutovsky P, and Dietmar E. Holm DE (2022) Potential use of tannin extracts as additives in semen destined for cryopreservation: a review. Animals, 1299):1130. doi: 10.3390/ani12091130

Cooper JW, Suman SP, Burdick KS, Sutovsky P, Lonergan SM, Lorenzen CL (2022) Color attributes and myoglobin chemistry exhibit relationships with tenderness and calpain-1 abundance in postmortem Longissmus lumborum muscles from Holstein heifers. Meat Sci., 189:108824. doi: 10.1016/j.meatsci.2022.108824.

Zigo M, Kerns K, Sen S, Essien C, Oko R, Xu D, and Sutovsky P (2022) Zinc is a master-regulator of sperm binding, motility, and metabolic modulation during porcine sperm capacitation Comms. Bio. (Nature Portfolio) 5:538.

Nogueira E, Tirpak F, Hamilton LEH, Zigo M, Kerns K, Sutovsky M, Kim JW, Volkmann D, Jovine L, Taylor JF, Schnabel RD, Sutovsky P (2022) A non-synonymous point mutation in a WD-40 domain repeat of EML5 leads to decreased bovine sperm quality and fertility. Frontiers in Cell Dev. Biol. 10:872740. doi: 10.3389/fcell.2022.872740

Shariff M, Hickl V, Juarez G, Di X, Kerns K, Sutovsky P, Bovin N, Miller DJ (2022) Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans. Sci. Rep., 12(1):6446. doi: 10.1038/s41598-022-10390-x

Anifandis G, Sutovsky P, Turek PJ, Chavez SL, Kunej T, Messini CI, Schon SB, Mavroforou A, Adashi EY, Krawetz SA (2022) Bioethics in human embryology: The double-edged sword of embryo research. Sys. Biol. Reprod. Med, 5:1-11. doi: 10.1080/19396368.2022.2052771.

Hamilton LE,Oko R, Miranda-Vizuete A, Sutovsky P (2022) Sperm redox system equilibrium: Implications for fertilization and male fertility. In: Oxidative Stress and Toxicity in Reproductive Biology and Medicine, Kesari KK, Roychoudhury, Editors. Springer Nature Switzerland AG. Adv. Exp. Med. Biol. 1358: 345-367.

Kaya A,Dogan S, Vargovic P, Kutchy NA, Ross P, Topper E, Oko R, van der Hoorn F, Sutovsky P and Memili E (2022) Sperm proteins ODF2 and PAWP as markers of fertility in breeding bulls. Cell Tissue Res., doi: 10.1007/s00441-021-03529-1

Carrillo- González DF, Lenis Sanín Y, Mohammed E, Sutovsky P, Drobnis E. (2022) Fisiología del espermatozoide “La célula más rápida del mundo” (Physiology of Spermatozoon “The fastest cell in the world) In:The World of the Testicle: Basic and Didactic Fundamentals of the Male Reproductive System, Yasser Yohan Lenis Sanín, editor; Chapter 4, National University of Colombia Medellín,.


Chen PR, Lucas CG, Cecil RF, Pfeiffer CA, Fudge MA, Samuel MS, Zigo M, Seo H, Lee D. Spate LD, Whitworth KM, Sutovsky P, Wells KD, Geisert RD, Prather RS (2021) Disrupting porcine glutaminase does not impair preimplantation development and elongation nor decrease mTORC1 activation. Biol. Reprod., In press.

Hamilton LE, Sutovsky P (2021) In: Oxidative stress and toxicity in reproductive biology and Medicine, Kesari KK, Roychoudhury, Editors. Springer Nature Switzerland AG. Pages: In press.

Hamilton LEH, Oko R, Sutovsky P (2021) Cellular and molecular events after ICSI in clinically relevant animal models. In: Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction. Palermo G and Nagy P, Editors. Cambridge University Press, In press.

Hamilton LE, Lion M, Aguila L, Suzuki J, Acteau G, Protopapas N, Wei Xu W, Sutovsky P, Baker M, Oko R (2021) Core histones are constituents of the perinuclear theca of murid spermatozoa. In. J. Mol. Sci., In press.

Sharif M, Kerns K, Sutovsky P, Bovin N, Miller DJ (2021) Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Reproduction, 161(4):449-457.

Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2021) Ligands and receptors involved in the sperm-zona pellucida interactions in mammals. Cells, In press.

Anifandis G, Tempest HG, Oliva R, Swanson GM, Simopoulou M, Eastey C, Primig M, Messini CI, Turek P , Sutovsky P, Ory S, Krawetz SA (2020) COVID-19 and human reproduction: A pandemic with serious circumstances. Syst. Biol. Reprod. Med., In press.

Klionsky D.J et al., (2020) Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, In press.

Mao J, Zigo M, Zuidema D, Sutovsky M, and Sutovsky P (2021) NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization. Biol. Reprod., 104(1):117-129.


Kerns K, Jankovitz J, Minton A, Kuster C, Robinson J, Sutovsky P (2020) Relationship between the length of sperm tail mitochondrial sheath and fertility traits in boars used for artificial insemination. Antioxidants, 9(11):1033. doi: 10.3390/antiox9111033

Ahlering P, Sutovsky M, Gliedt D, Branson K, Miranda Vizuete A, Sutovsky P (2020). Sperm content of TXNDC8 reflects sperm chromatin structure, pregnancy establishment, and incidence of multiple births after ART. Syst Biol Reprod Med. 66(5):311-321.

Tumova L, Zigo M, Sutovsky P, Sedmikova M, Postlerova P (2020) Is the ubiquitin-proteasome system involved in the degradation of porcine beta-microseminoprotein during sperm capacitation? Int. J. Mol. Sci., In press.

Benavides FG, Sutovsky P, López V, Kennedy C, Echevarría L (2020). Semen parameters of fertile guinea pigs (Cavia porcellus) collected by transrectal electroejaculation. Animals https://doi.org/10.3390/ani10050767

Kerns K, Sharif M, Zigo M, Xu W, Hamilton L, Sutovsky M, Ellersieck M, Drobnis EZ, Oko R, Miller D, Sutovsky P (2020) Sperm cohort-specific zinc signature acquisition and capacitation-induced zinc flux regulate sperm-oviduct and sperm-zona pellucida interactions. Int. J. Mol. Sci., Special Issue on Advances in Molecular Regulation of Spermatozoa Function, In press

Zigo M, Maňásková-Postlerová P, Zuidema D, Kerns K, Jonáková V, Tůmová L, Bubeníčková F, Sutovsky P (2020) Porcine model for the study of sperm capacitation, fertilization and male fertility. Cell Tissue Res., https://doi.org/10.1007/s00441-020-03181-1

Kelsey KM, Zigo M, Thompson WE, Kerns K, Gaurishankar Manandhar G, Sutovsky M, Sutovsky P (2020) Reciprocal surface expression of arylsulfatase A and ubiquitin in normal and defective mammalian spermatozoa. Cell Tissue Res.,In press.


(#200) Hamilton LE, Zigo M, Mao J, Xu W3, Sutovsky P, O’Flaherty C, Oko R (2019) GSTO2 isoforms participate in the oxidative regulation of the plasmalemma in eutherian spermatozoa during capacitation. Antioxidants, In press.

Štiavnická M, García-Álvarez O, Ulčová-Gallová Z, Sutovsky P, Abril-Parreño L, Dolejšová M, Řimnáčová H, Moravec J, Hošek P, Lošan P, Gold L, Fenclová T, Králíčková M, Nevoral J (2019) H3K4me2 accompanies chromatin immaturity in human spermatozoa: an epigenetic marker for sperm quality assessment. Systems Biol. Reprod. Med., In press DOI: 10.1080/19396368.2019.1666435

Zigo M, Manaskova-Postlerova P, Jonakova V, Kerns K, Sutovsky P (2019) Compartmentalization of the proteasome-interacting proteins during sperm capacitation. Sci. Rep. 9:12583

Zuidema D, Sutovsky P (2019) The domestic pig as a model for the study of mitochondrial inheritance. Cell Tissue Res., Special Issue on Animal Models, doi: 10.1007/s00441-019-03100-z

Huang Q, Liu H, Zeng J, Li W, Zhang S, Zhang L, Song S, Zhou T, Oko R, Sutoysky M, Sutoysky P, Pardi R, Hess RA, Zhang Z (2019) COP9 signalosome complex subunit 5, an IFT20 binding partner, is essential to maintain male germ cell survival and acrosome biogenesis. Biol. Reprod.,In press

Mordhorst B, Kerns K, Schauflinger M, Zigo M, Murphy S, Ross R, Wells K, Green J, Sutovsky P, Prather R  (2019) Pharmacologic treatment with CPI-613 and PS48 decreases mitochondrial membrane potential and increases quantity of autolysosomes in porcine fibroblasts. Sci Rep, 9:9417.

Zapata-Carmona H, Barón L, Zuñiga LM, Díaz ES, Kong M, Drobnis EZ, Sutovsky P, Morales PD (2019) The activation of the chymotrypsin-like activity of the proteasome is regulated by SACY/cAMP/PKA pathway and required for human sperm capacitation. Mol. Hum. Rep., In press doi: 10.1093/molehr/gaz037

St John JC, Sutovsky P (2019) Mitochondrial DNA: Fate of the Paternal Mitochondrial Genome. eLS – Wiley Online Library, In press.

Sutovsky P, Kerns K, Zigo M, Zuidema D (2019) Boar semen improvement through sperm capacitation management, with emphasis on zinc ion homeostasis.Theriogenology,137:50-55.

Hamilton LE, Suzuki J, Aguila L, Meinsohn M-C, Smith O, Protopapas N, Wei Xu3, Sutovsky P, Oko R (2019) Sperm-borne glutathione-s-transferase omega 2 accelerates the nuclear decondensation of spermatozoa during fertilization in mice. Biol. Reprod., 101:368-376.

Rodrigues WB, Silva AS, Silva JCB, Anache NA; da Silva KC, Cardoso CJT, Garcia WR, Sutovsky P, Nogueira E (2019) Timed Artificial Insemination plus heat II: Gonadorelin injection in cows with low estrus expression scores increased pregnancy in progesterone/estradiol-based protocol. Animal, 13:2313-2318

 Nogueira E, Silva MR, Silva JCB, U. P. G. Abreu UPG, Anache NA, Silva KC, Cardoso CJT, Sutovsky P, Rodrigues WB (2019) Timed Artificial Insemination plus heat I: Effect of estrus expression scores on pregnancy of cows subjected to progesterone-estradiol-based protocols. Animal, 13:2305-2312

Protopapas N, Hamilton L, Warkentin R, Xu W, Sutovsky P, and Oko R (2019) The post-acrosomal sheath and perforatorial regions of the perinuclear theca of rat spermatozoa share common developmental origins and protein constituents. Biol. Reprod.,100:1461-1472

Geisert RD, Sutovsky P, Lucy M, Bartol FF, Meyer AE (2019) Reproductive Physiology of Swine. In: Animal Agriculture: Challenges, Innovations, and Sustainability. Edited by Fuller W. Bazer, G. Cliff Lamb, and Guoyao Wu, Elsevier, In press.

Sutovsky P (2019) A mother’s gift. Adv. Anat. Embryol. Cell Biol., v-ix.

Zigo M, Jonakova V, Manaskova-Postlerova P, Kerns K, Sutovsky P (2019) Ubiquitin-proteasome system participates in the de-aggregation of spermadhesin and DQH protein during boar sperm capacitation. Reproduction, In press. doi: 10.1530/REP-18-0413.


Kerns K, Zigo M, Sutovsky P (2018) Zinc: A necessary ion for mammalian sperm fertilization competency. Int. J. Mol Sci., 19(12)

Mao J, O’Gorman C, Sutovsky M, Zigo M, Wells KD, Sutovsky P (2018) Ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52) is essential for preimplantation embryo development. BiolOpen, In press

Hamilton LE, Suzuki J, Acteau G, Shi M, Xu W, Meinsohn M-C, Sutovsky P, Oko R (2018)WBP2 shares a common location in mouse spermatozoa with WBP2NL and like its descendent is a candidate mouse oocyte activating factor. Biol. Reprod., In press.

Kerns K, Zigo M, Drobnis EZ, Sutovsky M, Sutovsky P (2018) Zinc ion flux during mammalian sperm capacitation. Nature Communications, 9(1):2061. doi: 10.1038/s41467-018-04523-y

Mao J, Pennington KA, Talton OO, Schulz LC, Sutovsky M, Yan L, Peter Sutovsky P (2018) In Utero and Postnatal Exposure to High Fat, High Sucrose Diet Suppressed Testis Apoptosis and Reduced Sperm Count. Scientific Reports 8:7622.

Sutovsky P (2018). Pig overview (male reproduction) In: Encyclopedia of Reproduction, Second edition, Vol. 1., B. Jegou & MK Skinner, Ed., Elsevier.

Taylor JF, Schnabel RD, Sutovsky P (2018) Identification of Genomic Variants Causing Sperm Abnormalities and Reduced Male Fertility, Anim Reprod Sci, In press. doi: 10.1016/j.anireprosci.2018.02.007.

Song W, Yi Y-J, Sutovsky M, Sutovsky P (2018) Mammalian cell-free system for the study of post-fertilization sperm mitophagy. In: AUTOPHAGY: Methods and Protocols. John Walker, Editor. Methods Mol Biol, Springer Nature, In press

Taylor JF, Schnabel RD, Sutovsky P (2018) Review: Genomics of bull fertility. Animal, 5:1-12 doi: 10.1016/j.anireprosci.2018.02.007

Sutovsky P (2018) Review: Sperm-oocyte interactions and their implications for bull fertility, with emphasis on ubiquitin-proteasome system. Animal, 12: 1-12 doi: 10.1017/S1751731118000253

Zigo M, Kerns K, Sutovsky M, Sutovsky P (2018). Modifications of the 26S proteasome during boar sperm capacitation. Cell Tissue Res., 372(3):591-601

Sutovsky P, Song W (2018) Post-fertilization sperm mitophagy: The tale of mitochondrial Eve and Steve. Reprod. Fert. Dev., 30:56-63.




Hamilton LE, Acteau G, Xu W, Sutovsky P, Oko RJ (2017)[1]The developmental origin and compartmentalization of glutathione-S-transferase Omega 2 isoforms in the perinuclear theca of Eutherian spermatozoa. Biol. Reprod., 97(4):612-621.

Adamkova K, Yi Y-J, Petr J, Zalmanova T, Hoskova K, Jelinkova P, Moravec J, Kralickova M, Sutovsky M, Sutovsky P, Nevoral J (2017) SIRT1-dependent modulation of methylation and acetylation of histone H3 on lysine 9 (H3K9) in the zygotic pronuclei improves porcine embryo development. J. Anim. Sci. Biotech., 8: 83.

Nevoral J, Sutovsky P, (2017) Epigenome modification and ubiquitin-dependent proteolysis during pronuclear development of the mammalian zygote: Animal models to study pronuclear development. In: Animal Models and Human Reproduction. Schatten H, Constantinescu GM, Editors, Wiley-Blackwell, pages 435-466.

Oko R., Aarabi M., Mao J, Balakier H, Sutovsky P (2017) Sperm specific WW-domain binding proteins. In: The Sperm Cell: Production, Maturation, Fertilization, Regeneration. Second Edition, DeJonge C, Barratt C, Eds., Cambridge University Press, Cambridge, UK, pages 157-176.





Song W, Yi Y-J, Sutovsky M, Meyers S, Sutovsky P (2016) The art and science of sperm mitophagy. Autophagy 12:2510-2511.

Štiavnická M, García Álvarez O, Nevoral J, Králíčková M, Sutovsky P (2016) Key features of genomic imprinting during mammalian spermatogenesis: perspectives for human assisted reproductive therapy. A review. Anat Physiol 6: 236. doi:10.4172/2161-0940.1000236

Song W, Yi Y-J, Sutovsky M, Meyers S, Sutovsky P (2016) Autophagy and ubiquitin-proteasome system contribute to sperm mitophagy after mammalian fertilization. Proc. Natl. Acad. Sci. USA 113:E5261-70

Kerns K, Morales P, Sutovsky P (2016) Regulation of sperm capacitation by the 26S proteasome: An emerging new paradigm in spermatology. Biol. Reprod., 94:1-9

Yin Y, Liu L, Lin C, Veith GM, Wang C, Sutovsky P, Zhou P, Ma L (2016) Cullin-Family Ubiquitin Ligase CUL4B is Required for Mouse Spermatogenesis. J. Bol. Chem., 291(13):6923-35

Song W, Sutovsky P (2016) Of Mitochondrial Eve and Steve. Mol. Reprod. Dev., 83: 5

Sutovsky P, Cupp AS, Thompson WE, Baker M (2016) Reproductive systems biology tackles global issues. Cell Tissue Res., 363(1):1-5




160. Hennings JM, Zimmer RL, Nabli H, Davis JW, Sutovsky P, Sutovsky M, Sharpe-Timms KL (2015) Improved murine blastocyst quality and development in a single culture medium compared to sequential culture media. Reproductive Sciences, 23(3):310-7

Nevoral J, Sutovsky P, Zamostna K, Petelak A, Hoskova K, Zalmanova T, Prokesova S, Jelinkova P, Marynikova V, Petr J (2015) SIRT1 as a key factor for histone code establishment in early embryo, from a perspective of assisted reproduction. Slovak J. Anim. Sci. 48:145-158.

Lin Y, Xu C, Sutovsky P, Wu D, Che L-Q, Fang Z-F, Xu S-Y, Ren B, Dong H-J (2015)

Impact of intrauterine growth restriction on long-term fertility in male pig model. Reprod. Fert. Dev., doi: 10.1071/RD15130

157. Sutovsky P (2015) New approaches to boar semen evaluation, processing and improvement. Dom. Anim. 50 Suppl. 2:11-19.

Dogan S, Vargovic P, Oliveira R, Belser RE, Kaya A, Moura A, Sutovsky P, Parrish J, Topper E, Memili E (2015) Sperm protamine status correlates to the fertility of breeding bulls. Biol. Reprod, 92(4): 1-9.

Ahlering P, Sutovsky P, (2015) Biomarker-based flow-cytometric semen analysis for male infertility diagnostics and clinical decision making in ART. In: Screening for the Single Euploid Embryo – Molecular Genetics in Reproductive Medicine, Eric Scott Sills, Editor, Springer Science+Media LLC, p. 33-51.

Sutovsky P, Aarabi M, Miranda-Vizuete A, Oko R (2015) Negative biomarker based male fertility evaluation: Sperm phenotypes associated with molecular-level anomalies. Asian J. Androl. 17, 554-60.

Aarabi M, Sutovsky P, Oko R (2015) Re: Is PAWP the ‘real’ sperm factor? Asian J. Androl. 17, 4-6. Invited Research Highlight – Male Fertility



152. Sutovsky P (2014) Posttranslational modifications-when the sperm cell meets the egg.  Adv Exp Med Biol. 759: v-vii.

Odhiambo JF, DeJarnette JM, Geary TW, Kennedy CE, Suarez SS, Sutovsky M, Sutovsky P (2014). Increased conception rates in beef cattle inseminated with nanopurified bull semen. Biol. Reprod., 91(4):97.

Aarabi M, Balakier H, Bashar S, Moskovtsev SI, Sutovsky P, Librach CL, Oko R. (2014) Sperm-derived WW domain-binding protein, PAWP, elicits calcium oscillations and oocyte activation in human and mouse. FASEB J., 28(10):4434-40., PMID: 24970390.

Petruska P, Capcarova M, Sutovsky P (2014) Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species. Turkish J Vet Anim Sci, 38:643-652. Special Issue on Recent Advances in Animal Reproduction, Keith Inskeep & Robert Dailey Editors,

Aarabi M, Balakier H, Bashar S, Moskovtsev SI, Sutovsky P, Librach CL, Oko R (2014) Sperm content of PAWP protein is related to assisted fertilization outcomes in patients undergoing ART. Fertil. Steril. 102(2):440-7

Yi Y-J, Sutovsky M, Song W-H, Sutovsky P (2014) Protein deubiquitination during oocyte maturation influences sperm function during fertilization, anti-polyspermy defense and embryo development. Reprod. Fert. Dev., 759:89-110.

Song W-H, Sutovsky P (2014) Regulation of mitochondrial inheritance by autophagy and ubiquitin-proteasome system: Implications for health, fitness and fertility. BioMed Research International, Special Issue on New Advances in Reproductive Biomedicine 2014:981867. doi: 10.1155/2014/981867

Mtango NR, Latham KE, Sutovsky, P. (2014) Deubiquitinating enzymes in oocyte maturation, fertilization and preimplantation embryo development. In: Sutovsky, P., Editor, Posttranslational Modifications in the Reproductive System, Springer Science +Business Media LLC, Adv Exp Med Biol. 2014;759:89-110

Kennedy CE, Krieger KB3, Sutovsky M, Xu W, Vargovič P, Didion BA, Ellersieck MR, Hennessy ME, Verstegen J, Oko R, Sutovsky P (2014) Protein expression pattern of PAWP in bull spermatozoa is associated with sperm quality and fertility following artificial insemination. Mol Reprod. Dev., 81(4): 436-49

Zimmerman S, Yi Y-J, Sutovsky M, van Leeuwen F, Conant G, Sutovsky P (2014) Identification and characterization of RING-finger ubiquitin ligase UBR7 in mammalian spermatozoa. Cell Tissue Res. 356:261-278.

Miles EL, Sutovsky P (2014) Sperm proteasome as a putative egg coat lysin in mammals. In: Sexual Reproduction in Animals and Plants, Hitoshi Sawada, Naokazu Inoue, and Megumi Iwano, Editors. Springer Science+Business Media LLC, pp. 441-463.


  1. 2013
  3. Baran V, Solc P, Kovarikova V, Rehak P, and Sutovsky P (2013) Polo-like kinase 1 is essential for the first mitotic division of mouse embryo. Mol. Reprod. Dev., 80:522-534.
  4. Wolff JN, Sutovsky P, Ballard JWO (2013). Mitochondrial DNA content of mature spermatozoa and oocytes in the genetic model Drosophila. Cell Tissue Res., 353:195-200.
  5. Miles EL, O’Gorman C, Zhao J, Samuel M, Walters E, Yi Y-J, Sutovsky M, Prather RS, Wells KD, Sutovsky P (2013) Transgenic pig carrying green fluorescent proteasomes. Proc. Natl. Acad. Sci. USA 110(16):6334-6339.
  6. Buckman CL, Ozanon C, Qiu J, Sutovsky M, Carafa JA, Rawe VY, Manandhar G, Miranda-Vizuete A, Sutovsky P (2013) Semen levels of spermatid-specific thioredoxin-3 correlate with pregnancy rates in ART couples. PLoS ONE, e61000. doi: 10.1371.
  7. Sutovsky P, Kennedy C. E. (2013) Biomarker-based nanotechnology for the improvement of reproductive performance in beef and dairy cattle. Indust. Biotech. 9: 24-30.
  8. Wolff JN, Nafisinia M, Sutovsky P, Ballard JWO (2013) Paternal transmission of mitochondrial DNA as an integral part of mitochondrial inheritance in metapopulations of Drosophila simulans. Heredity 110:57-62.
  10. 2012
  12. Sutovsky P (2012) The Real Pac-Man. Mol. Reprod. Dev., 79: 741
  13. Sutovsky P (2012) Cell biology solves mysteries of reproduction. Cell & Tissue Res., 349: 631-633.
  14. Yi, Y-J, Kennedy, C., Sutovsky, M., Sutovsky, P. (2012) Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa. PLoS ONE, 7(4):e34524.
  15. Aarabi, M., Yu, Y., Xu, W., Man, Y., Tse, M.Y., Pang, S.C., Yi, Y-J., Sutovsky, P., Oko, R. (2012)The Testicular and epididymal expression profile of PLCζ in mouse and human does not support its role as a sperm-borne oocyte activating factor. PLoS ONE, 7(3):e33496.
  16. Mtango, N., Sutovsky, M., VandeVoort, C. A., Latham, K.E., Sutovsky, P. (2012) Essential role of ubiquitin C-terminal hydrolases UCHL1 and UCHL3 in mammalian oocyte maturation. J. Cell. Physiol. 227: 2022-2029.
  17. Mtango, N., Sutovsky, M., Susor, A., Zhong, Z., Latham, K.E., Sutovsky, P. (2012) Essential role of maternal UCHL1 and UCHL3 in fertilization and preimplantation embryo development. J. Cell. Physiol. 227: 1592-1603
  18. Yi Y-J, Zimmerman SW, Manandhar G, Odhiambo JF, Kennedy C, Jonakova V, Manaskova-Postlerova P, Sutovsky M, Park C-S, Sutovsky P. (2012) Ubiquitin activating enzyme UBA1 is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization. Int. J. Androl., 35: 196-210.
  19. Nagyova E, Scsukova S, Nemcova L, Mlynarcikova A., Yi Y-J, Sutovsky M, Sutovsky P (2012). Inhibition of proteasomal proteolysis affects expression of extracellular matrix components and steroidogenesis in porcine oocyte-cumulus complexes. Dom. Anim. Endocrin., 42(1):50-62.
  22. 2011

Yi Y-J, Sung D Y, Millette C, Sutovsky M, Kennedy C, Sutovsky P, Thompson W, Thomas K (2011) Sperm GIRK2-containing K+ inward rectifying channels participate in sperm capacitation and fertilization. Systems Biol. Reprod. Med., 57(6):296-308.


Sutovsky P (2011) Sperm proteasome and fertilization. Reproduction, 142:1-14


Odhiambo JF, Sutovsky M, DeJarnette JM, Marshall C, Sutovsky P (2011) Technical note: Adaptation of ubiquitin-PNA based sperm quality assay for semen evaluation by a conventional flow cytometer and a dedicated platform for flow cytometric semen analysis. Theriogenology, 76 : 1168–1176

Zimmerman S.W., Manandhar G., Yi Y-J, Gupta S. K., Sutovsky M., Odhiambo J.F., Powell M. D., Miller, D.J.,Sutovsky P. (2011) Sperm proteasomes degrade sperm receptor on the egg zona pellucida during mammalian fertilization. PLoS ONE, 6(2):e17256


Sutovsky P, Oko R (2011) Spermatozoa: The good, the bad and the ugly. Mol. Reprod. Dev., 78(2):67


Yi Y-J, Zimmerman SZ, Sutovsky P (2011) Gamete binding and fusion. In: Cell Fusion: Regulation and Control. Lars-Inge Larsson, Editor, Springer Science + Business Media, LLC, New York, NY; p. 185-201

Kennedy, C., Ahlering, P., Rodriguez H., Levy S., Sutovsky, P. (2011) Sperm chromatin structure correlates with spontaneous abortion and multiple pregnancy rates in ART couples. Reprod. Biomed. Online., 22:271-276





Yi Y-J, Sutovsky P (2010) Gamete binding and fusion. In: Oocyte Maturation and Fertilization: A Long History for a Short Event (with foreword by Ryuzo Yanagimachi). Elisabetta Tosti, Raffaele Boni, Editors. Bentham Science Publishers Ltd., p.93-103

Whitworth KM, Spate LD, Li R, Rieke A, Sutovsky P, Green JA, Prather RS (2010) Activation method does not alter abnormal placental gene expression and development in cloned pigs.. Mol. Reprod. Dev., 77(12):1016-30

Sutovsky P, Lovercamp KL (2010) Molecular markers of sperm quality. In: Reproduction in Domestic Ruminants VII. Nottingham University Press, p. 247-256

Sutovsky P (2010) Establishing new paradigms while reexamining the old ones. Systems Biol. Reprod. Med., 56(5):331-3

Clark GF, Zimmerman S, Lafrenz DE, Young-Joo Yi Y-J, Sutovsky P (2010) Carbohydrate mediated binding and induction of acrosomal exocytosis in a sperm-somatic cell adhesion model. Biol. Reprod., 83(4):623-34

Yi, Y-J, Manandhar G, Sutovsky M, Zimmerman S, Jonáková V, van Leeuwen F, Oko R, Park C-S, Sutovsky P (2010) Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization. Cell & Tissue Res., 341(2):325-40.

Moore K,Lovercamp K, Feng D,Antelman J, Sutovsky M,Manandhar G,van Leyen K, Safranski T, Sutovsky P (2010) Altered epididymal sperm maturation and cytoplasmic droplet migration in the subfertile, male Alox15 mice. Cell & Tissue Res., 340(3):569-81.

Stilley JAW, Birt J.A., Nagel S.C., Sutovsky M., Sutovsky P., Sharpe-Timms K.L. (2010) Neutralizing TIMP1 restores fecundity in a rat model of endometriosis and treating control rats with TIMP1 causes anomalies in ovarian function and embryo development. Biol. Reprod., 83(2):185-94.

Susor A, Liskova L, Toralova T, Pavlok A, Pivonkova K, Karabinova P, Lopatarova M, Sutovsky P and Kubelka M (2010) Role of ubiquitin carboxyl-terminal hydrolase-L1 in anti-polyspermy defense of mammalian oocytes. Biol. Reprod., 82(6):1151-61.

Yi Y-J, Manandhar G, Sutovsky M, Jonáková V, Park C-S, and Sutovsky P (2010) Inhibition of 19S proteasomal regulatory complex subunit PSMD8 increases polyspermy during porcine fertilization in vitro. J. Reprod. Immunol., 84:154-163

Powell MD, Manandhar G, Spate L, Sutovsky M, Zimmerman S, Sachdev S, Hannink M, Prather RS, and Sutovsky P (2010) Discovery of putative oocyte quality markers by comparative ExacTag proteomics. Proteomics Clin. App., 4:337-251




Settivari RS, Evans TJ, Yarru LP, Eichen PA, Sutovsky P, Rottinghaus GE, Antoniou E, Spiers DE (2009) Effects of short-term heat stress on endophytic ergot alkaloid-induced alterations in rat hepatic gene expression. J. Anim. Sci., 87(10):3142-55

Buckman C, George TC, Friend S, Sutovsky M, Miranda-Vizuete A, Ozanon C, Morrissey P, Sutovsky P (2009) High throughput, parallel imaging and biomarker quantification of human spermatozoa by ImageStream flow cytometry. Systems Biol. Reprod. Med., 55(5-6):244-51

Sutovsky P (2009) Proteomic analysis of mammalian gametes and sperm-oocyte interactions. Soc. Reprod. Fert. Suppl. 66:103-16

Zimmerman S, Sutovsky P (2009) Recent progress in the study of the sperm proteasome during sperm capacitation and fertilization. J Reprod. Immunol., 83(1-2):19-25

Oko R, Sutovsky P (2009) Biogenesis of sperm perinuclear theca and its role in sperm functional competence and fertilization. J Reprod. Immunol., 83(1-2):2-7

Sutovsky P (2009) New insights into sperm-egg fusion in mammals. Exp. Rev. Molec. Med., 11:e11

Manandhar G, Miranda-Vizuete A, Pedrajas JR, Krause WJ, Zimmerman S, Sutovsky M, Sutovsky P. (2009) Peroxiredoxin-2 and peroxidase enzymatic activity of mammalian spermatozoa. Biol.Reprod. 80(6): 1168-77

Yi Y-J, Park C-S, Kim E-S; Song E-S, Jeong J-H, Sutovsky P (2009). Sperm-surface ATP in boar spermatozoa is required for fertilization: Relevance to sperm proteasomal function. Systems Biol. Reprod. Med., 55(2):85-96

Hao Y, Wax D, Zhong Z, Murphy C, Ross JW, Samuel M, Spate L, Dyce P, Li J, Sutovsky P, Prather RS (2009). The potential of the porcine skin-derived stem cells as donor cells for nuclear transfer. Cloning & Stem Cells, 11:101-109.

Whitworth KM, Li R, Spate LD, Wax DM, Rieke A, Whyte JJ, Manandhar G, Sutovsky M, Green JA, Sutovsky P, Prather RS. (2009) Method of oocyte activation affects cloning efficiency in pigs. Mol. Reprod. Dev., 76:490-500.

99. Stilley, J. A. W., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. (2009) Reduced fecundity in rats with surgically-induced endometriosis and their daughters: a role for tissue inhibitors of metalloproteinase-1. Reprod., 80:649-656

Sedó CA, Oko R, Sutovsky P, Chemes H, and Rawe VY (2009) Biogenesis of the sperm head perinuclear theca during human spermiogenesis. Fertil. Steril., 92(4):1472-1473.

Crimmins S, Sutovsky M, Chung P-C, Huffman A, Wheeler C, Swing D, Roth KA, Wilson JA,  Sutovsky P Wilson S  (2009) Transgenic rescue of ataxia mice reveals a male-specific sterility defect. Dev. Biol., 325(1):33-42



Viator J, Sutovsky P, Weight RM (2008) Detection of dilute sperm samples using photoacoustic flowmetry. Progress in Biomedical Optics and Imaging – Proceedings of SPIE, Vol. 6856, Article number 685607  

Han Z, Vassena R, Chi MM, Potireddy S, Sutovsky M, Moley KH, Sutovsky P, Latham KE (2008) Role of glucose in cloned mouse embryo development. Am. J. Physiol. Endocrinol. Metab. 295(4):E798-809

94. Antelman J, Manandhar G, Yi Y-J, Li R, Whitworth KM, Sutovsky M, Agca C,  Prather RS, and Sutovsky P (2008) Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development. J. Cell Physiol., 217:529-543

Sutovsky P (2010) Sperm capacitation, the acrosome reaction, and fertilization. In: Reproductive Endocrinology and Infertility: Integrating Modern Clinical and Laboratory Practice. Douglas Carrell and Matthew Peterson, Editors. Springer Science + Business Media, LLC, New York, NY; p. 389-421.

Baska KM, Manandhar G, Feng D, Agca Y, Tengowski MW, Sutovsky M, Yi Y-J, and Sutovsky P (2008). Mechanism of extracellular ubiquitination in the mammalian epididymis. J. Cell Physiol. 215:684-696.

Chakravarty S, Bansal P, Sutovsky P, and Gupta SK (2008). Role of proteasomal activity in the induction of acrosomal exocytosis by recombinant zona pellucida glycoproteins in human spermatozoa. Reprod. Biomed Online, 16(3):391-400.

Rawe VY, Díaz ES, Abdelmassih R, Wójcik C, Morales P, Sutovsky P, and Chemes HE (2008) The role of sperm proteasomes during sperm aster formation and early zygote development: Implications for fertilization failure in humans. Human Reprod., 23(3):573-580.

Yi Y-J, Nagyova E, Manandhar G, Prochazka R, Sutovsky M, Park C-S, Sutovsky P (2008)

Proteolytic activity of the 26S proteasome is required for the meiotic resumption, germinal vesicle breakdown, and cumulus expansion of porcine cumulus-oocyte complexes matured in vitro. Biol. Reprod., 78(1):115-126.



Pang P-C, Tissot B, Drobnis E, Sutovsky P, Morris HR, Sharpe-Timms K, Clark GF, and Dell A. (2007) Expression of bisecting type N-glycans and ligands for DC-SIGN on human sperm. Biol. Chem., 282(50):36593-36602.

Wu AC, Ph.D.; Sutovsky P,Xu W, van der Spoel AC, Platt FM, Oko RJ (2007) The postacrosomal assembly of sperm head protein, PAWP, is independent of acrosome formation and dependent on microtubular manchette transport. Dev. Biol., 312(2):471-483.

Yi Y-J, Manandhar G, Sutovsky M, Li R, Jonakova V, Oko R, Park C-S, Prather RS, Sutovsky P (2007) Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense. Biol. Reprod. 77, 780-793.

Lovercamp K.W., Safranski T.J., Fischer K.A., Manandhar G., Sutovsky M., Herring W., and Sutovsky P. (2007) High resolution light microscopic evaluation of boar semen quality and sperm cytoplasmic droplet retention in relationship with boar fertility. Arch. Androl: 53(4):219-28.

Wu ATH,* Sutovsky P,* Manandhar G, Xu W, Katayama M, Day BN, Park KW, Yi Y-J, Xi Y-W, Prather RS, and Oko R (2007) PAWP, a sperm specific WW-domain binding protein, promotes meiotic resumption and pronuclear development during fertilization. J. Biol. Chem., 282(16):12164-75. *PS and ATH Wu are listed as First Co-authors. Selected JBC Paper of the Week (top 1% of accepted papers).

Katayama M, Rieke A, Cantley T, Murphy C, Dowell L, Sutovsky P, Day BN (2007) Improved fertilization and embryo development resulting in birth of live piglets after intracytoplasmic sperm injection and in vitro culture in cysteine supplemented medium. Theriogenology, 67(4):835-47.


Tengowski MW, Feng D, Sutovsky M, Sutovsky  P (2007) Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats. Biol. Reprod. , 76(1):149-163.

Sutovsky P, Plummer W, Baska K, Peterman K, Diehl JR, Sutovsky M (2007) Relative levels of semen platelet activating factor-receptor (PAFr) and ubiquitin in yearling bulls with high content of semen white blood cells: Implications for breeding soundness evaluation. J. Androl., 28(1):92-108.

Yi Y-J, Manandhar G, Oko RJ, Breed WG, and Sutovsky P. (2007) Mechanism of sperm-zona pellucida penetration during mammalian fertilization: 26S proteasome as a candidate egg coat lysin. Soc Reprod Fertil Suppl. 63:385-408.



Lovercamp KW, Safranski TJ, Fischer KA, Manandhar G, Sutovsky M, Herring W, Sutovsky P (2006) Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology, 67(4):704-18.

78. Katayama, M., Zhong, Z., Lai, L., Sutovsky, P., Prather, R.S., Schatten, H. (2006). Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: Implications for developmental potential. Biol., 299(1):206-20.

Manandhar G, Feng D, Yi Y-J, Lai L, Letko J, Laurincik J, Sutovsky M, Salisbury JL, Prather RS, Schatten H & Sutovsky P (2006) Centrosomal protein centrin is not detectable during early pre-implantation development but reappears during late blastocyst stage in porcine embryos. Reproduction, 132(3):423-34.

Yu Y., Xu W., Yi Y-J, Sutovsky P., Oko R. (2006) The extracellular protein coat of the inner acrosomal membrane is involved in zona pellucida binding and penetration during fertilization: Characterization of its most prominent polypeptide (IAM38). Dev. Biol., 290:32-43.

Hao, Y., L. Lai, G.-S. Im, Z. Liu, D. Wax, M. Samuel, C.N. Murphy, P. Sutovsky, R.S. Prather. (2006). Developmental competence of porcine parthenogenetic embryos relative to embryonic chromosomal abnormalities. Mol. Reprod. Dev. 73:77-82.



Katayama M, Sutovsky P, Yang BS, Cantley T, Rieke A, Farwell R, Oko R & Day BN (2005) Increased disruption of sperm plasma membrane at sperm immobilization promotes dissociation of perinuclear theca from sperm chromatin after intracytoplasmic sperm injection in pigs. Reproduction, 130: 907-916.

Fischer KA, Van Leyen K, Lovercamp KW, Manandhar G, Sutovsky M, Feng D, Safranski T & Sutovsky P (2005) 15-Lipoxygenase (15-LOX) is a component of sperm cytoplasmic droplet. Reproduction, 130:213-222.

Sutovsky P, Manandhar G (2006) Mammalian Spermatogenesis and Sperm Structure: Anatomical and Compartmental Analysis. In: The Sperm Cell: Production, Maturation, Fertilization, Regeneration. DeJonge C, Barrat C, Eds., Cambridge University Press, Cambridge, UK, 1-30.

Ozanon C, Chouteau J, and Sutovsky P (2005) Clinical adaptation of the ubiquitin based sperm quality assay (SUTI): Relationship of sperm ubiquitination with sperm quality in gradient-purified semen samples from 93 men from a general infertility clinic population. Human Reprod., 20(8):2271-2278.

Manandhar G, Sutovsky P (2007). Comparative Histology and Subcellular Structure of Mammalian Spermatogenesis and Spermatozoa. In: Comparative Reproductive Biology. Schatten H, Ed., Iowa State Press, A Blackwell Publishing Company. p. 81-98.

Sutovsky P, Manandhar G, Laurincik J, Letko J, Caamano JN, Day BN, Lai L, Prather RS, Sharpe-Timms KL, Zimmer R, and Sutovsky M. (2005) Expression and proteasomal degradation of the major vault protein (MVP) in mammalian oocytes and zygotes.  Reproduction 129:269-279.

Manandhar D, Schatten H, Sutovsky P (2005). Centrosome reduction during gametogenesis and its significance. Biol Reprod. 72: 2-13.

Baska KM Sutovsky P, (2005) Protein modification by ubiquitination and is consequences for spermatogenesis, sperm maturation, fertilization and pre-implantation embryonic development. In: New impact on protein modifications in the regulation of reproductive system. Tokumoto, T, Ed., Research Signpost, Kerala; 83-114.

Tengowski M.W., Sutovsky P., Hedlund L.W., Guyot D.J., Burkhardt J.E., Thompson W. E., Sutovsky M., and Johnson G.A. (2005). Reproductive cytotoxicity is predicted by magnetic resonance microscopy and confirmed by ubiquitin-proteasome immunohistochemistry in a theophylline-induced model of rat testicular and epididymal toxicity. Microsc. & Microanalysis., 11:1-13.




Prather RS, Sutovsky P and Green JA. (2004) Nuclear remodeling and reprogramming in transgenic pig production. Exp. Biol. Med., 229: 1120-1126.

Sutovsky, P., Manandhar G., McCauley, T.C., Caamaño JN, Sutovsky, M., Thompson WE, and Day, B.N. (2004). Proteasomal interference prevents zona pellucida penetration and fertilization in mammals: Biol Reprod., 71, 1625–1637

JiménezA, Zu W, Rawe VY, Pelto-Huikko M, Flickinger CJ, Sutovsky P, Gustafsson J-A, Oko R and Antonio Miranda-Vizuete A. (2004) Spermatocyte/spermatid-specific thioredoxin-3, a novel Golgi apparatus-associated thioredoxin, is a specific marker of aberrant spermatogenesis. J. Biol. Chem. 279(33):34971-34982.

Tovich PR, Sutovsky P and Oko RJ (2004) Novel aspect of perinuclear theca assembly revealed by immunolocalization of non-nuclear somatic histones during bovine spermiogenesis. Biol. Reprod., 71(4):1182-94.

Thompson WE, Asselin E, Branch A, K. Stiles JK, Sutovsky P, Lai L, Im G-S, Prather RS, Isom SC, Rucker III E and Tsang BK (2004) Regulation of prohibitin expression during follicular development and atresia in the mammalian ovary. Biol. Reprod., 71(1):282-290.

Sutovsky P, Prather RS (2004) Nuclear remodeling after SCNT: A contractor’s nightmare. Trends Biotech., 22(5):205-8

Sutovsky P, Hauser R, Sutovsky M (2004) Increased levels of sperm ubiquitin correlate with  semen quality in  men from an andrology laboratory clinic population. Human Reprod. 19:1-11.

              578 Miranda-Vizuete A, Sadek CM, Jiménez A, Krause WJ, Sutovsky P and Oko R (2004) The mammalian testis-specific thioredoxin system. Antioxid Redox Signal. 6:25-40

Sutovsky P, van Leyen K, McCauley T, Day BN, and Sutovsky M (2004).               Degradation of the paternal mitochondria after fertilization: Implications for heteroplasmy, ART and mtDNA inheritance. Reprod. Biomed. Online 8, 24-33.



Miranda-Vizuete A, Tsang K, Yu Y, Alberto Jiménez A, Pelto-Huikko M, Flickinger CJ, Sutovsky P, and Oko R (2003). Cloning and developmental analysis of murid spermatid-specific thioredoxin-2 (Sptrx-2), a novel sperm fibrous sheath protein and autoantigen. J Biol Chem., 278:44874-44885.

Thompson WE, Ramalho-Santo J, and Sutovsky P (2003). Ubiquitination of prohibitin in mammalian sperm mitochondria: Possible roles in the regulation of mitochondrial inheritance and sperm quality control. Biol. Reprod., 69:254-260

Sutovsky, P., Manandhar, G., Wu, A., and Oko, R. (2003) Interactions of the sperm perinuclear theca with the oocyte: Implications for oocyte activation, anti-polyspermy defense and assisted reproduction. Microsc. Res. Tech., 61:362-378. (Invited Article).

Sutovsky, P., McCauley, T.C., Sutovsky, M., and Day, B.N. (2003). Early degradation of paternal mitochondria in domestic pig (Sus scrofa) is prevented by selective proteasomal inhibitors lactacystin and MG 132. Biol. Reprod., 68:1793-1800.

Sutovsky P. 2003. Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone. Micros. Res. Tech., 61: 88-102 (Invited contribution)

Sutovsky P. (2004) Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa and zygotes In : Methods In Molecular Biology, vol 253: Germ Cell Protocols: Vol. 1Sperm and Oocyte Analysis. (Heide Schatten, Editor), Human Press, Totowa, NJ pp. 59-77.

50. Rawe, V.Y. , Brugo Olmedo, S., Nodar, F.N., Ponzio, R., Sutovsky, P. (2003) Abnormal assembly of annulate lamellae and nuclear pore complexes coincides with fertilization arrest at pronuclear stage of human zygotic development. Human Reprod., 18, 576-582.

Sutovsky P, Turner RM, Hameed S, Sutovsky M (2003) Differential ubiquitination of stallion sperm proteins: Possible implications for infertility and reproductive seasonality. Biol. Reprod., 68, 688-698




Rawe, V.Y., Brugo Olmedo, S., Benmusa, A.,Shiigi, S. M., Chemes, H.E., and Sutovsky, P. 2002. Sperm Ubiquitination in Patients with Dysplasia of the  Fibrous Sheath.  Human Reprod., 17:2119-2127.

Sutovsky, P., Neuber, E., and Schatten, G. (2002). Ubiquitin-Dependent, Sperm Quality Control Mechanism Recognizes Spermatozoa with DNA Defects, as Revealed by Dual Ubiquitin-TUNEL Assay. Reprod. Dev., 61: 406-413.



Sutovsky, P., Motlik, J., Neuber, E., Pavlok, A., et al., (2001) Accumulation of the proteolytic marker peptide ubiqutin in the trophoblast of mammalian blastocysts. Cloning & Stem Cells 3, 157-161.

Oko R, Aul R, Wu A, Sutovsky P. 2001. The sperm head skeleton. In: Andrology in the 21st century. Robaire B, Chemes H, Morales CR, Editors. Medimond Publishing Co., Englewood NJ,pp.37-51

Sutovsky, P., Moreno, R., Ramalho-Santos, J., Dominko, T., Thompson, W. E., and Schatten, G. (2001). A Putative, Ubiquitin-Dependent Mechanism for The Recognition and Elimination of Defective Spermatozoa in the Mammalian Epididymis. J. Cell Sci. 114, 1665-1675

Sutovsky, P., Terada, Y., and Schatten, G. (2001) Ubiquitin-based sperm assay for the diagnosis of male factor infertility. Human Reprod. , 16, 250-258



Sutovsky, P.*, et al., cited as Ramalho-Santos, J.*, Sutovsky, P.*, Simerly, C., Oko, R., Wessel, G., Hewitson, L., and Schatten, G. (2000) ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications. Human Reprod. 15, 2610-2620; *P.S>. And J. R.-S. contributed equally to this study and are thus acknowledged as “first co-authors” in the paper.

Sutovsky, P., Moreno, R., Ramalho-Santos, J., Dominko, T., Simerly, C., and Schatten, G. (2000) Ubiquitinated sperm mitochondria, selective proteolysis and the regulation of mitochondrial inheritance in mammalian embryos. Biol. Reprod. 63, 582-590

Ramalho-Santos J., Moreno, R. D., Sutovsky, P., Chan,A. W.-S., Hewitson, L. C., Wessel, G. M., Simerly, C. R., and Schatten, G. (2000) SNAREs in mammalian sperm: Possible implications for fertilization. Dev. Biol.  223, 54-69.

Moreno, R., Ramalho-Santos, J., Sutovsky, P., Chan, E. L. K., and Schatten G (2000) Vesicular traffic and golgi aparatus dynamics during mammalian spermatogenesis: Implications for acrosome architecture. Biol. Reprod.,  63, 89-98

Terada, Y., Luetjens, C. M., Sutovsky, P., and Schatten, G. (2000) Atypical decondensation of the sperm nucleus, delayed replication of the male genome and sex chromosome positioning following intracytoplasmic human sperm injection into golden hamster eggs: Does ICSI itself introduce chromosomal anomalies? Fertil. Steril., 74(3), 454-460

Sutovsky, P., and Schatten, G. (2000) Paternal Contributions to the mammalian zygote: fertilization after sperm-egg fusion. Int. Rev. Cytol. 195, 1-65 (Invited article)



Sutovsky, P., Moreno, R., Ramalho-Santos, J., Dominko, T., Simerly, C., and Schatten, G. (1999) Ubiquitin tag for sperm mitochondria. Nature 402, 371-372

Simerly, C., Zoran, S. S., Payne, C., Dominko, T., Sutovsky, P., Navara, C. S., Salisbury, J. L., and Schatten, G. (1999) Biparental inheritance of G- tubulin during human fertilization: Molecular reconstitution of functional zygotic centrosomes in inseminated human oocytes and in cell-free extracts nucleated by human sperm. Mol. Biol. Cell 10, 2955-2969

Sutovsky, P., Ramalho-Santos, J., Moreno, R. D., Oko, R., Hewitson, L., and Schatten, G. (1999) On-Stage Selection of Single Round Spermatids Using a Vital, Mitochondrion-Specific Fluorescent Probe MitoTrackerTM and High Resolution Differential Interference Contrast (DIC) Microscopy. Human Reprod., 14, 2301-2312

Hewitson, L., Simerly, C., Sutovsky, P., Dominko, T., Takahashi, D., and Schatten, G. (1999) The fate of sperm components within the egg during fertilization. In: The Male Gamete: From Basic Science to Clinical Applications. C. Gagnon, Ed., Cache River Press, Vienna, IL.

Hewitson L., Dominko T., Takahashi D., Ramalho-Santos J., Sutovsky P., Fanton J., Jacob D., Monteith D., Neuringer M., Battaglia D., Simerly C., and Schatten., G. (1999) Births of ICSI monkeys: Unique checkpoints during the first cell cycle of fertilization. Nature Med. 5, 431-433

Sutovsky, P., Manandhar, G., and Schatten, G. (1999) Biogenesis of the Centrosome During Mammalian Gametogenesis and Fertilization. Protoplasma 206, 249-262



Sutovsky, P., and Schatten, G. (1998) Structural aspects of mammalian fertilization: New discoveries and Inspirations from the work of Daniel Szöllösi. Reprod. Nutr. Dev. 38, 629-641

Srsen, V., Kalous, J., Nagyova, E., Sutovsky, P., King, W. A., and Motlik, J. (1998) Effects of follicle-stimulating hormone, bovine somatotrophin and okadaic acid on cumulus expansion and nuclear maturation of Blue fox (Alopex lagopus) oocytes in vitro. Zygote 6, 299-310

Hewitson, L., Simerly, C., Sutovsky, P., Dominko,T., and Schatten, G. (1998) Molecular reconstitution and inheritance of the centrosome during fertilization: Implications for fertility. In: Lauria, A., et al. (eds.): Gametes: Development and Function. Serono Symposia, Rome, pp. 371-392

Sutovsky, P., Simerly, C, Hewitson, L., and Schatten, G. (1998) Assembly of nuclear pore complexes and annulate lamellae promotes normal pronuclear development in fertilized mammalian oocytes. J. Cell Sci. 111, 2841-2854

Manandhar, G., Sutovsky, P., Joshi, H. C., Stearns, T., and Schatten, G. (1998) Centrosome reduction during mouse spermiogenesis. Dev. Biol. 203, 424-434

25. Simerly, C., Hewitson, L., Sutovsky, P., Takahashi, D., and Schatten, G. (1998) The role of centrosome and cytoskeleton during human fertilization: Implications for the new frontiers in infertility treatments. In: Treatment of Infertility: The New Frontiers. Communication Media for education

24. Schatten, G., Hewitson, L., Simerly, C., Sutovsky, P., and Huszar, G. (1998) Cell and molecular biological challenges of ICSI: A.R.T. before science? Law, Med. & Ethics, 26: 5-13

Sutovsky P., Flechon J.E. (1998) Cytoskeletal reorganization during cumulus expansion. In: Microscopy of Reproduction and Development. A Dynamic Approach. P. M. Motta, Ed., pp. 115-124.




Sutovsky P, Oko R, Hewitson L, and Schatten G. (1997) The removal of the sperm perinuclear theca and its association with the bovine oocyte surface during fertilization. Dev. Biol., 188: 75-84

Sutovsky P., and Schatten G. (1997) Depletion of glutathione during oocyte maturation reversibly blocks the decondensation of the male pronucleus and pronuclear apposition during fertilization. Biol. Reprod. 56:1503-1512

Sutovsky P., Tengowski M.W., Navara C.S., Zoran S.S., and Schatten G., (1997) Mitochondrial sheath movement and detachment in mammalian, but not non-mammalian, sperm induced by disulfide bond reduction. Mol. Reprod. Dev. 47: 79-86

Simerly CR, Hewitson LC, Sutovsky P, Navara CS, Schatten G (1997) Predicted forms of male infertility as a result of disorders in the paternally inherited human centrosome. Proc. IX World Congr. Hum. Reprod.

Simerly CR, Hewitson LC, Sutovsky P, Schatten G (1997) The inheritance, molecular dissection and reconstruction of the human centrosome during fertilization: consequences for infertility. In: Barrat C., de Jonge C., Mortimer D., Parinaud J. (eds), Genetics of human male fertility. EDK Press, Paris, p. 147-163




Simerly CR, Haawisto AJ, Hewitson LC, Sutovsky P, Navara CS, Schatten G (1996) The molecular dissection and reconstitution of the centrosome during human fertilization: Implications for infertility. S. Afr. J. Sci. 92: 548-557

Motlik J., Sutovsky P., Kalous J., Kalab P., Kubelka M., and Schultz R. M. (1996) Co-culture with pig membrana granulosa cells modulates the activity of cdc2 and MAP kinase in maturing cattle oocytes. Zygote 4: 247-256

15. Navara C.S., Hewitson L., Simerly C.R., Sutovsky P., and Schatten G. (1996) The implications of a paternally derived centrosome during human fertilization: Consequences for reproduction and the treatment of male factor infertility. J. Reprod. Imun. 37: 39-49

Hewitson LC, Simerly CR, Sutovsky P, Navara CS, Tengowski MW, Schatten G. (1996) Advanced imaging of human fertilization. In: Visual atlas of sperm function for AR (Human).  H. Sathannathan, Ed., 155-173

Sutovsky P., Hewitson L., Simerly C.R., Schatten G. (1996) Molecular medical approaches for alleviating infertility and understanding assisted reproductive technologies. Proc. As. Amer. Phys. , 108/6: 432-443

Sutovsky P, Hewitson LC, Simerly CR, Tengowski MW, Navara CS, Haavisto AJ, Schatten G. (1996) Intracytoplasmic sperm injection (ICSI) for Rhesus monkey fertilization results in unusual chromatin, cytoskeletal, and membrane events, but eventually leads to pronuclear development and sperm aster assembly. Human Reprod.  11:1703-1712

Sutovsky P, Navara CS, Schatten G (1996). The fate of the sperm mitochondria and the incorporation, conversion and disassembly of the sperm tail structures during bovine fertilization in vitro. Biol. Reprod. 55: 1195-1205

Hewitson LC, Simerly CR, Tengowski MW, Sutovsky P, Navara CS, Haavisto AJ, Schatten G. (1996) Microtubule and DNA configurations during Rhesus intracytoplasmic sperm injection: successes and failures. Biol. Reprod. 55:271-280



Sutovsky P, Flechon JE, Pavlok A (1995) F-actin is involved in control of bovine cumulus expansion. Mol. Reprod. Dev. 41: 521-529



Sutovsky P, Motlik J (1994) Cumulus expansion in mammals: An interplay between cumulus granulosa cell cytoskeleton, extracellular matrix and oocyte secretion. ARTA J. 6: 304-315

Sutovsky P, Flechon JE, Pavlok A (1994) Microfilaments, microtubules and intermediate filaments fulfil differential roles in gonadotropin induced expansion of bovine cumulus oophorus. Reprod. Nutr. Develop.  34: 415-425



Nagyová E, Kalous J, Sutovsky P, Motlík J; (1993) Is cAMP   decrease essential for resumption of meiosis in mouse oocytes? Reprod. Nutr. Develop. 33: 419-428

 Sutovsky P, Fléchon J E, Fléchon B, Motlík J, Peynot N, Chesné P, Heyman Y,; (1993) Dynamic changes of gap junctions and  cytoskeleton during in vitro culture of cattle oocyte cumulus complexes.  Biol. Reprod., 49: 1277-1287

Kanka J, Fléchon J E, Sutovsky P; (1993) Changes in RNA     synthesis and poly(A) content of early rabbit and sheep embryos. Reprod. Nutr. Develop. 33: 465-474

 Sutovsky P, Jelínková L, Antalíková L, Motlík J; (1993)     Ultrastructural cytochemistry of the nucleus and nucleolus   in growing rabbit oocytes. Biol. Cell  77: 173-180

Kalous J, Sutovsky P, Rimkevicová Z, Shioya Y, Lie B L, Motlík J; (1993) Pig membrana granulosa cells prevent resumption of meiosis in cattle oocytes. Mol. Reprod. Dev.  34: 58-64




Procházka R, Kanka J, Sutovsky P, Fulka J, Motlík J; (1992) Development of pronuclei in pig oocytes activated by a single    electric pulse. J. Reprod. Fert. 96: 725-734